| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nalls, Madison P (15)
|
MADSC |
2:27.18 |
2:34.54 |
7.36 |
| 2 |
Rippon, Caylene A (16)
|
MAHAC |
2:30.94 |
2:34.78 |
3.84 |
| 3 |
Hester, Kelly N (15)
|
MADSC |
2:38.50 |
2:35.08 |
-3.42 |
| 4 |
Olson, Rachel L (14)
|
MADSC |
2:30.27 |
2:42.36 |
12.09 |
| 5 |
Breitbach, Alaina M (13)
|
MADSC |
2:33.64 |
2:44.34 |
10.70 |
| 6 |
Weller, Allison H (13)
|
MAHAC |
2:48.52 |
2:48.82 |
0.30 |
| 7 |
McCloskey, Grace M (14)
|
MAHAC |
2:52.85 |
2:51.40 |
-1.45 |
| 8 |
Yoder, Katelyn A (13)
|
MADSC |
3:02.40 |
3:00.79 |
-1.61 |
| 9 |
Tardibuono, Taylor K (14)
|
MALAC |
3:35.99 |
3:01.60 |
-34.39 |
| 10 |
Yeager, Rebecca E (14)
|
MAHAC |
2:59.52 |
3:02.91 |
3.39 |
| 11 |
Nakano, Tomomi (16)
|
MAHAC |
3:01.81 |
3:04.35 |
2.54 |
| 12 |
Kramer, Nina E (14)
|
MALAC |
2:52.06 |
3:06.19 |
14.13 |
| 13 |
Pedersen, Ella S (13)
|
MAHAC |
3:20.03 |
3:10.07 |
-9.96 |
| 14 |
Yun, Michelle J (13)
|
MAHAC |
3:19.19 |
3:10.97 |
-8.22 |
| 15 |
Correll, Isabella C (14)
|
MAHAC |
3:23.17 |
3:13.22 |
-9.95 |
| 16 |
Eichman, Olivia A (14)
|
MAHAC |
3:18.21 |
3:14.65 |
-3.56 |
| 17 |
Bova, Alessia (13)
|
MAHAC |
3:22.61 |
3:16.93 |
-5.68 |
| 18 |
Kriner, Ember J (15)
|
MALAC |
3:18.11 |
3:23.78 |
5.67 |
| 19 |
Fitterer, MayaRose P (13)
|
MAHAC |
|
3:24.48 |
- |
| 20 |
Grubb, Kirsten A (13)
|
MAHAC |
|
3:30.71 |
- |